In vitro activity of mastoparan-AF alone and in combination with clinically used antibiotics against multiple-antibiotic-resistant Escherichia coli isolates from animals

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• 作者：Chun-Hsien Lin^a ; Roger F. Hou^a ; Ching-Lin Shyu^b ; ^c ; Wei-Yau Shia^b ; Chuen-Fu Lin^d ; Wu-Chun Tu^a ; ^{wctu@dragon.nchu.edu.tw}
• 关键词：Mastoparan-AF ; Antimicrobial resistance gene ; Antimicrobial activity ; Antibiotic ; E. coli ; Synergism
• 刊名：Peptides
• 出版年：2012
• 期刊代码：59_01969781
• 类别：neu
• 出版时间：July, 2012
• 卷：36
• 期：1
• 页码：114-120
• 文件大小：302 K

摘要

The in vitro activity of mastoparan-AF, an amphipathic antimicrobial peptide isolated from the hornet venom of Vespa affinis, alone and in combination with various clinically used antibiotics, was investigated against 21 Escherichia coli isolates/strains. Most E. coli isolates tested were detected containing multiple-antimicrobial resistance genes. Antimicrobial activity of mastoparan-AF was measured by MIC, MBC, time-kill kinetic assay and chequerboard titration method. Mastoparan-AF exhibited potent antimicrobial activity against most multiple-antibiotic-resistant E. coli isolates at the concentrations ranging from 4 to 16 渭g/ml. Combination studies showed that mastoparan-AF acts synergistically with certain antibiotics, i.e., cephalothin or gentamicin, against some multiple-antibiotic-resistant E. coli isolates. In conclusion, mastoparan-AF alone or in combination with other antibiotics could be promising as alternatives for combating multiple-antibiotic-resistant E. coli in future clinical applications.