Immunological patterns identifying disease course and evolution in multiple sclerosis patients
- 作者：Roberto Furlan ; Marco Rovaris ; Filippo Martinelli Boneschi ; Mohsen Khademi ; Alessandra Bergami ; Maira Gironi ; Michela Deleidi ; Federica Agosta ; Diego Franciotta ; Elio Scarpini et al.
- 关键词：Multiple sclerosis ; Chemokines ; Cytokines ; Real-time RT-PCR ; Multivariate analysis
- 刊名：Journal of Neuroimmunology
- 出版年：2005
- 期刊代码：32_01655728
- 类别：neu
- 出版时间：2005
- 卷：165
- 期：1-2
- 页码：192-200
- 文件大小：158 K
摘要
Reliable, and easy to measure, immunological markers able to denote disease characteristics in multiple sclerosis (MS) patients are still lacking. We applied a multivariate statistical analysis on results obtained by measuring–by real-time RT-PCR–mRNA levels of 25 immunological relevant molecules in PBMCs from 198 MS patients. The combined measurement of mRNA levels of IL-1β, TNF-α, TGF-β, CCL20 and CCR3 was able to distinguish MS patients from healthy individuals. CXCR5, CCL5, and CCR3 combined mRNA levels identify primary progressive MS patients while TNF-α, IL-10, CXCL10 and CCR3 differentiate relapsing MS patients. Our results indicate that multi-parametric analysis of mRNA levels of immunological relevant molecules in PBMCs may represent a successful strategy for the identification of putative peripheral markers of disease state and disease activity in MS patients.