Co-inoculation of Dactylaria brochopaga and Monacrosporium eudermatum affects disease dynamics and biochemical responses in tomato (Lycopersicon esculentum Mill.) to enhance bio-protection against Meloidogyne incognita

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• 作者：Udai B. Singh^a ; ^{udaiars.nbaim@gmail.com} ; ^{udaibhu1@gmail.com} ; Asha Sahu^b ; Nisha Sahu^c ; R.K. Singh^a ; Renu^d ; Ratna Prabha^d ; Dhananjaya P. Singh^d ; B.K. Sarma^a ; M.C. Manna^b
• 关键词：Nematophagous fungi ; Dactylaria brochopaga ; Monacrosporium eudermatum ; Root gall ; Total phenolic compounds ; Phenylalanine ammonia lyase
• 刊名：Crop Protection
• 出版年：2012
• 期刊代码：28_02612194
• 类别：abs
• 出版时间：May, 2012
• 卷：35
• 期：Complete
• 页码：102-109
• 文件大小：1284 K

摘要

The nematode trapping ability of nematophagous fungi Dactylaria brochopaga and Monacrosporium eudermatum to colonize tomato root and parasitize and kill root-knot nematode Meloidogyne incognita was evaluated, along with the capability of the fungi to induce the accumulation of defence-related biomolecules in tomato plants under the greenhouse and field conditions. Co-inoculation of D.聽brochopaga Dp-5 and M.聽eudermatum Mv-1 significantly reduced root-knot disease in tomato (89.63%) and increased the accumulation of total chlorophyll (125.34, 140.53 and 152.67聽mg聽g^鈭? fresh wt.), total phenolic compounds (TPC) (37.40, 48.32 and 59.63聽渭g of gallic acid equivalent), and phenylalanine ammonia lyase (PAL) activity (58.45, 69.05 and 74.57聽mM cinnamic acid聽h^鈭?聽g^鈭? fresh wt.) after 10, 20 and 30 days of inoculation, respectively, in the greenhouse. However, in the field experiments, the total chlorophyll content in leaves of M.聽incognita infected tomato plants co-inoculated with D.聽brochopaga Dp-5 and M.聽eudermatum Mv-1 (152.70聽mg聽g^鈭? fresh wt.) was higher than the plants treated with D.聽brochopaga Dp-5 or M.聽eudermatum Mv-1 individually (130.87 and 144.73聽mg聽g^鈭? fresh wt., respectively) and M.聽incognita treated plants (19.47聽mg聽g^鈭? fresh wt.). TPC and PAL activity were found to be higher in tomato leaves co-inoculated with D.聽brochopaga Dp-5 and M.聽eudermatum Mv-1 (62.90聽渭g of gallic acid equivalent and 79.33聽mM cinnamic acid聽h^鈭?聽g^鈭? fresh wt., respectively) than the plants treated with D.聽brochopaga Dp-5 or M.聽eudermatum Mv-1 individually, whereas in M.聽incognita inoculated and control plants, the induction of these two compounds was found to be very low after 30 days of inoculation. The results of this study showed that these fungi reduced root-knot disease and enhanced accumulation of defence related biomolecules in tomato plants and has potential to serve as a biocontrol agents against root-knot nematodes.