A quantum dot-based optical immunosensor for human serum albumin detection

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• 作者：Meng-Che Tu^a ; Yun-Tzu Chang^a ; Yu-Ting Kang^a ; Hwan-You Chang^b ; Pin Chang^c ; Tri-Rung Yew^a ; ^{tryew@mx.nthu.edu.tw}
• 关键词：Human serum albumin ; Quantum dot ; Optical immunosensor ; Photodiode ; 3-Aminopropyltriethoxysilane ; Diode laser
• 刊名：Biosensors and Bioelectronics
• 出版年：2012
• 期刊代码：12_09565663
• 类别：ch
• 出版时间：15 April, 2012
• 卷：34
• 期：1
• 页码：286-290
• 文件大小：813 K

摘要

In this study, a CdSe/ZnS quantum dot (QD)-based immunosensor using a simple optical system for human serum albumin (HSA) detection is developed. Monoclonal anti-HSA (AHSA) immobilized on 3-aminopropyltriethoxysilane (APTES)-modified glass was used to capture HSA specifically. Bovine serum albumin (BSA) was used to block non-specific sites. The solution, containing AHSA-QD complex prepared by mixing biotinylated polyclonal anti-HSA and streptavidin coated QD, was used to conjugate with the HSA molecules captured on AHSA/BSA/APTES-modified glass for the modification of HSA with QD. A simple optical system, comprising a diode laser (405 nm), an optical lens, a 515-nm-long pass filter, and an Si-photodiode, was used to detect fluorescence and convert it to photocurrent. The current intensity was determined by the amount of QD specifically conjugated with HSA, and was therefore HSA-concentration-dependent and could be used to quantify HSA concentration. The detection limit of the pure QD solution was 鈭?.5 脳 10^鈭?2 M, and the detection limit for the CdSe/ZnS QD-based immunosensor developed in this study was approximately 3.2 脳 10^鈭? mg/ml. This small optical biosensing system shows considerable potential for future applications of on-chip liver-function detection.