Involvement of aldolase A in X-ray resistance of human HeLa and UV^r-1 cells

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• 作者：Jun Lu ; Toshikazu Suzuki ; Mamoru Satoh ; Shiping Chen ; Takeshi Tomonaga ; Fumio Nomura ; Nobuo Suzuki
• 关键词：Aldolase A ; Proteomic analysis ; Radio-resistance ; Colony survival
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2008
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：9 May 2008
• 卷：369
• 期：3
• 页码：948-952
• 文件大小：303 K

摘要

To find novel proteins involved in radio-resistance of human cells, we searched for nuclear proteins, whose expression levels alter after X-ray irradiation in HeLa cells, using agarose fluorescent two-dimensional differential gel electrophoresis following mass spectrometry. We identified 6 proteins, whose levels were increased in nuclei 24 h after irradiation at 5 Gy, including aldolase A. Nuclear aldolase A levels increased twofold after the irradiation, however, total aldolase A levels did not change. When the expression of aldolase A was suppressed by its specific siRNA, sensitization of the suppressed cells to X-ray-induced cell death was observed. In addition, UV^r-1 cells with higher aldolase A expression exhibited lower sensitivity to X-ray-induced cell death than the parental RSa cells with lower aldolase A expression. These results suggest that aldolase A may play a role in the radio-response of human cells, probably in nuclei, in addition to its glycolytic role in the cytosol.