Antioxidant S-allylcysteine prevents gentamicin-induced oxidative stress and renal damage
- 作者：Maldonado ; Perla D. ; Barrera ; Diana ; Rivero ; Isabel ; Mata ; Rachel ; Medina-Campos ; Omar N. ; et. al.
- 关键词：S-allylcysteine ; Gentamicin ; Garlic ; Acute renal failure ; Superoxide dismutase ; Glutathione peroxidase ; Oxidative stress ; Free radicals
- 刊名：Free Radical Biology and Medicine
- 出版年：2003
- 期刊代码：105_08915849
- 类别：med
- 出版时间：August 1, 2003
- 卷：35
- 期：3
- 页码：317-324
- 文件大小：210 K
摘要
Acute renal failure (ARF) is a major complication of gentamicin (GM) treatment, which is effective against gram-negative infections. Since experimental evidence suggests a role of reactive oxygen species (ROS) in GM-induced ARF, in this work we studied the effect of a garlic-derived compound, S-allylcysteine (SAC), which is a free radical scavenger, on GM-induced nephrotoxicity. In rats treated with GM (70 mg/kg/12 h/4 days/s.c.), ARF was evident by the: (i) decrease in creatinine clearance and increase in blood urea nitrogen, (ii) decrease in blood glutathione peroxidase (GPx) activity and increase in urinary excretion of N-acetyl-β-D-glucosaminidase and total protein, and (iii) necrosis of proximal tubular cells. These alterations were prevented by SAC treatment (250 mg/kg/i.p. 24 h before the first dose of GM and 125 mg/kg/12 h/4 days along GM-treatment). Furthermore, SAC prevented the GM-induced oxidative stress (protein carbonyl groups) and the decrease in manganese superoxide dismutase (Mn-SOD), GPx, and glutathione reductase (GR) activities in renal cortex. In conclusion, SAC ameliorates the GM-induced ARF by a mechanism related, at least in part, to its ability to decrease oxidative stress and to preserve antioxidant enzymes activity in renal cortex.