Suppressive effects of destruxin B on hepatitis B virus surface antigen gene expression in human hepatoma cells
- 作者：Chen ; Hua-Chien ; Chou ; Chen-Kung ; Sun ; Chang-Ming ; Farn Yeh ; Sheau
- 关键词：Fungus metabolite ; Cyclodepsipeptide ; Antiviral agent ; Gene regulation
- 刊名：Antiviral Research
- 出版年：1997
- 期刊代码：7_01663542
- 类别：imm
- 出版时间：May, 1997
- 卷：34
- 期：3
- 页码：137-144
- 文件大小：526 K
摘要
Destruxin B, a cyclodepsipeptide was originally identified as a plant pathogen from the fungus, Alternaria brassicae. We examined the antiviral activity of destruxin B and found that it suppresses the expression of the hepatitis B viral surface antigen (HBsAg) gene in human hepatoma Hep3B cells which carry an integrated viral gene in its chromosome. In contrast, destruxin B shows no cytotoxic effect on the viability of the cells. Furthermore, it can be shown that destruxin B can reversibly suppress HBsAg production by Hep3B cells in a concentration-dependent manner with EC50 of 0.5 μM. Northern blot analysis indicates that the suppression of HBsAg gene expression by destruxin B is mainly at the mRNA level. Destruxin B not only suppresses the endogenously expressed HBsAg in the Hep3B cells but also suppresses the HBsAg produced either from the stable transfected HBV DNA in another human hepatoma HuH-7 cell line which carry no endogenous HBV genome. These results suggest that destruxin B may have future potential for development as a specific anti-HBV drug.