Biomonitoring marine habitats in reference to antibiotic resistant bacteria and ampicillin resistance determinants from oviductal fluid of the nesting green sea turtle, Chelonia mydas
- 作者：S.N. Al-Bahry ; snbahry@squedu.om ; M.A. Al-Zadjali ; I.Y. Mahmoud ; A.E. Elshafie
- 关键词：Biomonitoring ; Pollution ; Bacteria ; Ampicillin resistance ; Oviductal fluid ; Green turtles
- 刊名：Chemosphere
- 出版年：2012
- 期刊代码：15_00456535
- 类别：env
- 出版时间：June, 2012
- 卷：87
- 期：11
- 页码：1308-1315
- 文件大小：463 K
摘要
During the egg-laying process, oviductal fluid was collected using a non-invasive procedure from the cloacal vent of the green turtles. Forty-two independent isolates of antibiotic-resistant bacteria from 11 genera were obtained from 20 turtles during nesting. The dominant isolate was Citrobacter (52.4%), followed by Pseudomonas, Proteus, Enterobacter, Salmonella, Escherichia coli, Shigella, Edwardsiella, Morganella, Providencia and Arcomobacter. Most of the isolates were resistant to ampicillin. Ampicillin-resistant isolates showed variations in their resistance for the following classes of 尾-lactamases: extended-spectrum 尾-lactamases (EBSLs), AmpC type 尾-lactamases C (AmpC), and screen-positive 尾-lactamase. None of the isolates produced metallo 尾-lactamase. Some ampicillin-resistant genes were detected by multiplex polymerase chain reaction (PCR) only. Inhibitor based test (IBT) categorized some isolates as AmpC 尾-lactamase producers. 尾-Lactamase genes were detected from a few strains. The sequencing of those genes revealed the presence of cephamycinase (CMY) and AmpC 尾-lactamases. The oviductal fluid was used in this study as a source of bacterial antibiotic-resistant determinants for biomonitoring marine turtles exposed to contaminated effluents. This data can be of value in understanding the decline of this endangered species as a result of exposure to marine pollution which is threatening their survival.