Purification and characterization of maltooligosaccharide-forming 伪-amylase from moderately halophilic Marinobacter sp. EMB8

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• 作者：Sumit Kumar ; S.K. Khare ; ^{skhare@rocketmail.com} ; ^{skkhare@chemistry.iitd.ac.in}
• 关键词：Halophiles ; 伪-Amylase ; Maltooligosaccharides ; Marinobacter sp. ; Solvent stable
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：July, 2012
• 卷：116
• 期：Complete
• 页码：247-251
• 文件大小：218 K

摘要

Maltooligosaccharides especially maltotriose and maltotetraose producing amylases are highly desirable for application in bread making and other food industries. A maltotriose and maltotetraose producing amylase from moderately halophilic Marinobacter sp. EMB8 is described. Under optimized culture conditions, 48.0 IU/mL amylase was obtained. The enzyme was purified to homogeneity by ultrafiltration, DEAE cellulose and Sephadex G-75 column chromatography with 52%yield and 76-fold purification. It was a monomeric protein of 72 kDa. The amylase had many novel features viz. stability up to 20%NaCl, 80 掳C temperature, pH 6.0-11.0 and in wide range of organic solvents at high concentrations. The enzyme efficiently hydrolyzed starch into maltooligosaccharides rich in maltotriose and maltotetraose. These novel properties make the Marinobacter sp. amylase a potentially useful enzyme.