Investigation on chemical composition, anticholinesterase and antioxidant activities of extracts and essential oils of Turkish Pinus species and pycnogenol

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• 作者：Osman Ustun^a ; Fatma Sezer Senol^a ; Mine Kurkcuoglu^b ; Ilkay Erdogan Orhan^a ; ^c ; ^{iorhan@gazi.edu.tr} ; ^{ilkay.orhan@emu.edu.tr} ; Murat Kartal^d ; Kemal Husnu Can Baser^b ; ^e
• 关键词：Antioxidant activity ; Cholinesterase inhibition ; Essential oil ; Pinaceae ; Pinus ; Pycnogenol
• 刊名：Industrial Crops and Products
• 出版年：2012
• 期刊代码：56_09266690
• 类别：abs
• 出版时间：July, 2012
• 卷：38
• 期：Complete
• 页码：115-123
• 文件大小：682 K

摘要

In the current work, the acetone, ethyl acetate, and ethanol extracts and essential oils of the twigs and needles of five Turkish Pinus species; P. brutia, P. halepensis, P. nigra, P. pinea, and P. sylvestris along with pycnogenol (the bark extract of P. pinaster) were examined for their inhibitory effects against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) at 200 渭g mL^鈭?. Their antioxidant activity was tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and N,N-dimethyl-p-phenylendiamine (DMPD) radical scavenging, metal-chelation, and ferric-reducing antioxidant power (FRAP) assays. Total phenol and flavonoid contents of the extracts were determined by Folin-Ciocalteau and AlCl₃ reagents, respectively. GC-MS technique was applied for compositional profile of the essential oils of the tested species. Accordingly, the best AChE and BChE inhibition was caused by the twig essential oil (83.91 卤 3.95%) and the needle ethanol extract (82.47 卤 5.57%) of P. halepensis, respectively. Pycnogenol also caused a remarkable inhibition against AChE (63.33 卤 0.22%) and BChE (83.67 卤 0.22%). The extracts and essential oils usually showed a strong activity in the antioxidant assays. Pycnogenol exhibited a potent antioxidant effect and had the richest total phenol content. In the essential oils, 伪- and 尾-pinene were identified the major components. These results reveal that the Pinus species and pycnogenol are the potential sources of active metabolites with cholinesterase and antioxidant properties.