B cell mitogenic activity of house dust mite, Dermatophagoides farinae, antigens
- 作者：Fujii ; Seiji ; Ono ; Kazuhisa ; Shigeta ; Seiko ; Oka ; Satoru ; Jyo ; Tomohiko ; Yamashita ; Uki
- 关键词：Dermatophagoides farinae ; B cell proliferation ; Ig production ; Purified mite antigen
- 刊名：Immunology Letters
- 出版年：1996
- 期刊代码：115_01652478
- 类别：med
- 出版时间：January, 1996
- 卷：49
- 期：1-2
- 页码：37-42
- 文件大小：552 K
摘要
The effect of mite antigens on murine and human lymphocytes was studied in vitro. Antigens prepared from Dermatophagoides farinae feces and bodies stimulated normal murine spleen cells to proliferate in a dose-dependent manner. The responder cells are B cells, because the response was reduced by the treatment of spleen cells with anti-immunoglobulin antibody and complement, but not with anti-Thy 1 antibody and complement. Furthermore, nylon column-purified T cells did not respond. The stimulation of B cells with mite antigens was not due to the contamination of lipopolysaccharide, a representative B cell mitogen, because spleen cells which are low responders to lipopolysaccharide could respond to mite antigens. These antigens induced not only proliferative response of murine B cells, but also immunoglobulin production. By gel-filtration column chromatography, the active fractions were eluted around the molecular weight of 150-155 kDa. Furthermore, mite antigens also stimulated human B cells to proliferate and to produce immunoglobulin. All these results suggest that mite antigens are a potent B cell mitogen and this activity might concern the induction of allergic reaction.