Enhanced expression of B7.2 (CD86) by percutaneous sensitization with house dust mite antigen
- 作者：Santa ; Kazuki ; Watanabe ; Kenji ; Nakada ; Tsutomu ; Kato ; Hiroyuki ; Habu ; Sonoko ; Kubota ; Shunichiro
- 关键词：Atopic dermatitis (AD) ; House dust mite antigen ; Th1/Th2 ; IgE ; IL-4 ; IFN-γ ; B7.1 (CD80) ; B7.2(CD86)
- 刊名：Immunology Letters
- 出版年：2003
- 期刊代码：115_01652478
- 类别：med
- 出版时间：January 2, 2003
- 卷：85
- 期：1
- 页码：5-12
- 文件大小：283 K
摘要
House dust mite antigen is a well-known allergen in the pathogenesis of atopic dermatitis (AD), a chronic relapsing inflammatory skin disease. We evaluated the AD model mice sensitized with house dust mite antigen and observed a Th2-dominant immune response. In this experiment, BALB/c mice were sensitized percutaneously with house dust mite antigen three times with 7 days interval after skin barrier disruption. A remarkable infiltration of polymorphonuclear granulocytes and monocytes in the cutis was observed in mice treated with this antigen, high serum IgE levels and IL-4 mRNA expression in local lymph node cells was also observed. CD19+ B cell numbers overturned to CD4+ helper T cells. In these mice, there was significant increase of B7.2 (CD86) expression on CD19+ B cells. These results indicate that house dust mite antigen sensitizes BALB/c mice and skews their Th1/Th2 balance toward Th2.