Insights into the pathophysiology of the antiphospholipid syndrome provided by atomic force microscopy

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• 作者：Anthony S. Quinn^a ; Xiao-Xuan Wu^b ; Jacob H. Rand^b ; Douglas J. Taatjes^a ; ^{douglas.taatjes@uvm.edu}
• 关键词：Atomic force microscopy ; Antiphospholipid syndrome ; Annexin A5 ; 尾2 glycoprotein I ; Hydroxycholoroquine ; Thrombosis ; Placenta ; Recurrent pregnancy losses
• 刊名：Micron
• 出版年：2012
• 期刊代码：37_09684328
• 类别：ph
• 出版时间：August, 2012
• 卷：43
• 期：8
• 页码：851-862
• 文件大小：2983 K

摘要

The antiphospholipid syndrome (APS) is an enigmatic autoimmune disorder in which patients present with thrombosis and/or recurrent pregnancy losses together with laboratory evidence for the presence of autoantibodies in the blood that recognize proteins that bind to anionic phospholipids - the most important of which is 尾₂-glycoprotein I (尾₂GPI). Earlier, we hypothesized that the clinical manifestations arise from antibody-induced disruption of a two-dimensional anticoagulant crystal shield, composed of annexin A5, present on placental trophoblast plasma membranes. Accordingly, we reasoned that a high resolution imaging technology, such as atomic force microscopy could be used to investigate such molecular interactions at high resolution in a non-fixed hydrated environment. This review will focus on the contribution of this technique to the elucidation of the mechanism of APS.