MAPK7 and MAP2K4 as prognostic markers in osteosarcoma

详细信息	查看全文 | 推荐本文 |

• 作者：Francine Tesser-Gamba ; MSc^a ; ^b ; ^{francine.tesser@yahoo.com.br} ; Antonio Sergio Petrilli ; MD ; PhD^a ; ^{sergiopetrilli@graacc.org.br} ; Maria Teresa de Seixas Alves ; MD ; PhD^a ; ^c ; ^{mtseixas@patologia.epm.br} ; Reynaldo Jesus Garcia Filho ; MD ; PhD^d ; ^{jesusgarcia.dot@epm.br} ; Yara Juliano ; PhD^e ; ^{yjuliano@unisa.br} ; Sí ; lvia Regina Caminada Toledo ; PhD^a ; ^b ; ^{silviatoledo@graacc.org.br}
• 关键词：Osteosarcoma (OS) ; Gene expression ; OS markers ; MAPK7 ; MAP2K4
• 刊名：Human Pathology
• 出版年：2012
• 期刊代码：140_00468177
• 类别：med
• 出版时间：July, 2012
• 卷：43
• 期：7
• 页码：994-1002
• 文件大小：496 K

摘要

| Figures/TablesFigures/Tables | ReferencesReferencesml version="1.0" encoding="UTF-8"?>

Summary

Osteosarcoma is a class of cancer originating from the bone, affecting mainly children and young adults. Cytogenetic studies showed the presence of rearrangements and recurrent gains in specific chromosomal regions, indicating the possible involvement of genes located in these regions during the pathogenesis of osteosarcoma. These studies investigated expression of 10 genes located in the chromosomal region involved in abnormalities in osteosarcoma, 1p36, 17p, and chromosome 19. The purpose of this study was to investigate the expression profile of genes located in regions involved in chromosomal rearrangements in osteosarcoma. We used quantitative real-time polymerase chain reaction to investigate the expression of 10 genes located in 1p36.3 (m>MTHFRm>, m>ERRFI1m>, m>FGRm>, m>E2F2m>), 17p (m>MAPK7m>, m>MAP2K4m>), and chromosome 19 (m>BBC3m>, m>FOSBm>, m>JUNDm>, and m>RRASm>), in 70 samples taken from 30 patients (30 prechemotherapy, 30 postchemotherapy, and 10 metastases specimens) and 10 healthy bones as a control sample. The most interesting results showed a strong association between the expression levels of m>MAPK7m> and m>MAP2K4m> genes and clinical parameters of osteosarcoma. Overexpression of these genes was significantly associated to a poor response to treatment (m>Pm> = .0001 and m>Pm> = .0049, respectively), tumor progression, and worse overall survival (m>Pm> = .0052 and m>Pm> = .0085, respectively), suggesting that m>MAPK7m> and m>MAP2K4m> could play an important role in osteosarcoma tumorigenesis. Thus, these genes could be good markers in assessing response to treatment and development of osteosarcoma.