Copper-mediated DNA photocleavage by a tetrapyridoacridine (tpac) ligand
- 作者：Marta Gonzá ; lez-Á ; lvarez ; Marí ; a-Selma Arias ; Marí ; a-José ; Ferná ; ndez ; Lourdes Gude ; Antonio Lorente ; Gloria Alzuet ; Joaquí ; n Borrá ; s
- 关键词：Tetrapyridoacridine ; Copper ; DNA ; Photonuclease
- 刊名：Bioorganic and Medicinal Chemistry Letters
- 出版年：2008
- 期刊代码：10_0960894x
- 类别：ch
- 出版时间：1 June 2008
- 卷：18
- 期：11
- 页码：3286-3290
- 文件大小：423 K
摘要
We have focused our interest on the tetrapyridoacridine ligand tetrapyrido[3,2-a:2′,3′-c:3′′,2″-h: 2
,3-j]acridine (tpac), as a model system for the preparation of novel copper-based artificial nucleases. The complex of copper(II)–tpac cleaves supercoiled pUC18 plasmid DNA in an oxidative manner by photoactivation with visible light, exhibiting maximum cleaving efficiency at 1:2 metal–ligand stoichiometric ratio. We propose an interaction of the copper–tpac complex with DNA through both major and minor grooves and a photocleavage mechanism via the formation of hydroxyl radicals and singlet oxygen or singlet oxygen-like species.
,3-j]acridine (tpac), as a model system for the preparation of novel copper-based artificial nucleases. The complex of copper(II)–tpac cleaves supercoiled pUC18 plasmid DNA in an oxidative manner by photoactivation with visible light, exhibiting maximum cleaving efficiency at 1:2 metal–ligand stoichiometric ratio. We propose an interaction of the copper–tpac complex with DNA through both major and minor grooves and a photocleavage mechanism via the formation of hydroxyl radicals and singlet oxygen or singlet oxygen-like species.