• 作者：Josep Julve^a ; Cristina Muñ ; oz Garcí ; a^a ; David Santos^a ; Francisco Blanco-Vaca^a ; ^b ; ^c ; Joan Carles Escolà ; -Gil^a ; ^c ; ^{jescola@santpau.cat}
• 关键词：ApoA-II ; ApoC ; HDL ; Lipoproteí ; na lipasa ; Triglicé ; ridos
• 刊名：Cl铆nica e Investigaci贸n en Arteriosclerosis
• 出版年：2010
• 期刊代码：pca88_02149168
• 类别：med
• 出版时间：September-October 2010
• 卷：22
• 期：5
• 页码：192-197
• 文件大小：205 K

Introduction

Apolipoprotein (apo)A-II is the second most abundant HDL protein but its function remains largely unknown. Studies in humans and genetically-modified mice have demonstrated a role for apoA-II in triglyceride metabolism. The main objective of this study was to evaluate the relationship between apoA-II and HDL apolipoprotein composition, as well as HDL-mediated lipoprotein lipase (LPL) coactivation and plasma triglyceride concentration.

Methods

Eleven-hour fasting blood samples were taken from 32 healthy volunteers. Anthropometric data and lipid and apolipoprotein parameters were analyzed. HDL isolated by ultracentrifugation was incubated in the presence of a triolein-based emulsion and bovine LPL. In 14 of these volunteers, an additional blood sample was taken 3 h after breakfast.

Results

ApoA-II concentration was directly correlated with plasma triglycerides (R=0.55, p<0.05) and inversely correlated with the HDL-apoC-II+apoE/apoC-III ratio (R=鈭?.43, p<0.05). ApoA-II was also inversely correlated with HDL-mediated LPL coactivation (R=鈭?.35, p<0.05). ApoA-II concentration was directly correlated with plasma triglycerides 3 h after the fat-loading test (R=0.58, p<0.05).

Conclusion

These results show that HDL-apoA-II levels play a crucial role in triglyceride catabolism and suggest that, at least in part, this is due to modulation of LPL activity.