纬-Sitosterol from Acacia nilotica L. induces G2/M cell cycle arrest and apoptosis through c-Myc suppression in MCF-7 and A549 cells

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• 作者：Shenbagamoorthy Sundarraj^a ; Ramar Thangam^a ; ^b ; Vellingiri Sreevani^a ; Krishnasamy Kaveri^b ; Palani Gunasekaran^b ; Shanmugam Achiraman^c ; Soundarapandian Kannan^a ; ^{sk_protein@buc.edu.in}
• 关键词：Acacia nilotica ; Traditional medicine Africa and Asia ; Human cancer cells ; MTT assay ; Apoptosis ; G2/M arrest
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 期刊代码：26_03788741
• 类别：pha
• 出版时间：14 June, 2012
• 卷：141
• 期：3
• 页码：803-809
• 文件大小：789 K

摘要

Ethnopharmacological relevance

Acacia nilotica is widely distributed in Asia. In India, it occupies an important place in the indigenous system of medicine against anti-inflammatory, antioxidant, cancers, and/or tumors.

Aim of the study

The purpose of this study is to investigate the inhibitory effect of Acacia nilotica leaves extract and 纬-Sitosterol on cell proliferation, the apoptotic effect and cell cycle arrest in breast and lung cancer cells.

Materials and methods

GC-MS and HPLC were used to determine the chemical constituents of this extract and 纬-Sitosterol respectively. Human MCF-7 and A549 cell lines were treated with Acacia nilotica extract and 纬-Sitosterol. Cell viability was determined by MTT assay. Cell proliferation was determined by BrdU incorporation assay. Apoptosis was detected by cell morphologic observation through AO/EtBr staining, cell cycle analysis, and immunoblot analysis on the expression of protein associated with cell cycle arrest.

Results

Experimental results of bioactive compound analysis indicate that 纬-Sitosterol, bioactive ingredients of Acacia nilotica extract. The IC₅₀ value of extract on MCF-7 and A549 cancer cells was 493.3 卤 15.2 and 696.6 卤 11.5 渭g/ml, respectively. Acacia nilotica extract and 纬-Sitosterol were inhibited the cell proliferation by 54.34 卤 1.8 and 42.18 卤 3.9%for MCF-7 and 58.26 卤 1.5 and 44.36 卤 3.05%for A549 cells. The percentage of apoptotic cells observed in the MCF-7 and A549 cell lines were increased to 42.46 and 36.8%of extract; 46.68 and 43.24%for 纬-Sitosterol respectively. Flow cytometric analysis results demonstrate that cells were arrested at the G2/M phase and decrease the c-Myc expression.

Conclusions

This study demonstrates in vitro results, which support the ethnomedical use of 纬-Sitosterol against cancer. Experimental results of this study suggest that 纬-Sitosterol exerts potential anticancer activity through the growth inhibition, cell cycle arrest and the apoptosis on cancer cells.