A novel disintegrin protein from Naja naja venom induces cytotoxicity and apoptosis in human cancer cell lines in vitro

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• 作者：Ramar Thangam^a ; ^b ; ^{thangam1985@yahoo.co.in} ; Palani Gunasekaran^b ; Krishnasamy Kaveri^b ; Gnanaiah Sridevi^c ; Shenbagamoorthy Sundarraj^a ; Manickam Paulpandi^a ; Soundarapandian Kannan^a ; ^{sk_protein@buc.edu.in}
• 关键词：N. naja ; Disintegrin ; DNA fragmentation ; Apoptosis ; G0/G1 cycle arrest in vitro
• 刊名：Process Biochemistry
• 出版年：2012
• 期刊代码：29_13595113
• 类别：bio
• 出版时间：August, 2012
• 卷：47
• 期：8
• 页码：1243-1249
• 文件大小：1457 K

摘要

Animal venoms and toxins are potential bioresources that have been known to mankind as a therapeutic tool for more than a century through folk and traditional medicine. The purified 鈥渄isintegrin protein鈥?(64 kDa) from the venom of the Indian cobra snake (Naja naja) exhibited cytotoxic effects of various types of human cancer cell lines such as breast cancer (MCF-7), lung cancer (A549) and liver cancer (HepG2). In vitro cytotoxicity, DNA fragmentation, an apoptotic assay and a cell cycle analysis were performed to evaluate the anticancer activity of disintegrin against the above cell lines. The IC₅₀ value of disintegrin was determined to be 2.5 卤 0.5 渭g/mL, 3.5 卤 0.5 渭g/mL, and 3 卤 0.5 渭g/mL for the MCF-7, A549 and HepG2 cell lines respectively. Moreover, the increased distribution of G0/G1 and S phase led to decreased populations of cells in the G2/M phase of MCF-7, HepG2 and A549 cells.