Genotyping of Chlamydia trachomatis from endocervical specimens of infertile Mexican women

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• 作者：Maria de Jesú ; s De Haro-Cruz^a ; Irma Deleó ; n-Rodriguez^a ; Marcos R. Escobedo-Guerra^b ; Marcela Ló ; pez-Hurtado^b ; Gabriel Arteaga-Troncoso^b ; Federico J. Ortiz-Ibarra^c ; Fernando M. Guerra-Infante^a ; ^b ; ^{fguerra_96@yahoo.com}
• 关键词：Genotypes of Chlamydia trachomatis ; Infertility ; lymphogranuloma venereum ; L2 genovar ; Sexual transmitted disease
• 刊名：Enfermedades Infecciosas y Microbiolog铆a Cl铆nica
• 出版年：2011
• 期刊代码：pca153_0213005X
• 类别：med
• 出版时间：February 2011
• 卷：29
• 期：2
• 页码：102-108
• 文件大小：1009 K

摘要

Introduction

It has been reported in several countries that m>Chlamydia trachomatism> genotypes D, E, and F are the ones more frequently associated with urogenital infections. In Mexico, the prevalence of serovars and genotypes is unknown.

Material and Methods

One hundred and fifty-two endocervical swabs were collected from infertile women to test for m>C. trachomatism>. The PCR-based RFLP and automated-sequencing methods of m>ompAm> gene was used to identify the m>C. trachomatism> genotypes. Sequences of 891 pb obtained were aligned with currently available chlamydial sequences from GenBank to identify the corresponding genotype.

Results

Twenty-four women with infertility (15.8%) were positive for m>C. trachomatism>. According to the RFLP and nucleotide sequences results the most prevalent ompA genotype corresponded to serovar F (n = 13 [54.2%]), followed by serovars E (n = 2 [8.7%]), G (n = 2 [8.7%]), K (n = 2 [8.7%]) and LGV (n = 2 [8.7%]), while serovars D, H and Ia were less prevalent (all n = 1 [4.2%]). None of the patients who were positive to genovar L2 had symptoms of lymphogranuloma venereum (LGV). Nucleotide sequences analysis showed a new genovariant of L2, which was different to L2b to L2f. Mutation points were observed in VS1 domain of m>Omp Am>.

Conclusions

In this study the most common genotypes were F. Furthermore, the L2 genovariants were demonstrated in infertile women without signs and symptoms of LGV disease. Presence of point mutations in L2 genotype sequences were seen by which there is a need for further research in order to identify new L2 genetic variants that exist in Latin America.