A novel stimulus artifact removal technique for high-rate electrical stimulation
- 作者：Leon F. Heffer ; James B. Fallon
- 关键词：Stimulus artifact ; Artifact removal ; Electrical stimulation ; Electrophysiology ; Auditory nerve
- 刊名：Journal of Neuroscience Methods
- 出版年：2008
- 期刊代码：33_01650270
- 类别：neu
- 出版时间：30 May 2008
- 卷：170
- 期：2
- 页码：277-284
- 文件大小：441 K
摘要
Electrical stimulus artifact corrupting electrophysiological recordings often makes the subsequent analysis of the underlying neural response difficult. This is particularly evident when investigating short-latency neural activity in response to high-rate electrical stimulation. We developed and evaluated an off-line technique for the removal of stimulus artifact from electrophysiological recordings. Pulsatile electrical stimulation was presented at rates of up to 5000 pulses/s during extracellular recordings of guinea pig auditory nerve fibers. Stimulus artifact was removed by replacing the sample points at each stimulus artifact event with values interpolated along a straight line, computed from neighbouring sample points. This technique required only that artifact events be identifiable and that the artifact duration remained less than both the inter-stimulus interval and the time course of the action potential. We have demonstrated that this computationally efficient sample-and-interpolate technique removes the stimulus artifact with minimal distortion of the action potential waveform. We suggest that this technique may have potential applications in a range of electrophysiological recording systems.