Exposure to the environmental estrogen bisphenol A differentially modulated estrogen receptor-α and -β immunoreactivity and mRNA in male mouse testis
- 作者：Takao ; Toshihiro ; Nanamiya ; Wakako ; Pournajafi Nazarloo ; Hossein ; Matsumoto ; Reiko ; Asaba ; Koichi ; et. al.
- 关键词：Bisphenol A ; Estrogen receptors ; Immunohistochemistry ; RT-PCR ; Testis
- 刊名：Life Sciences
- 出版年：2003
- 期刊代码：62_00243205
- 类别：imm
- 出版时间：January 24, 2003
- 卷：72
- 期：10
- 页码：1159-1169
- 文件大小：253 K
摘要
We examined the effects of bisphenol A (0.5 μg/ml or 50 μg/ml) in the drinking water on estrogen receptor (ER) α and β proteins and mRNA in the testis of young mice following 8-weeks of oral administration of bisphenol A utilizing immunohistochemistry and semiquantitative reverse transcription polymerase chain reaction amplification (RT-PCR). ERβ was clearly localized in the nuclei of spermatogonia and/or spermatocytes. ERβ immunopositive cell numbers per testis section were significantly decreased in the 50 μg/ml bisphenol A-treated group compared with control and the 0.5 μg/ml bisphenol A-treated group. The number of ERα positive cells in the testis was significantly lower than ERβ positive cells in control group. ERα immunopositive cell numbers per testis section were markedly increased in the 50 μg/ml bisphenol A-treated group compared with the control and the 0.5 μg/ml bisphenol A-treated group. ERβ mRNA expression was significantly decreased in the 50 μg/ml bisphenol A-treated group compared with the control and the 0.5 μg/ml bisphenol A-treated group. In contrast, ERα mRNA expression was markedly increased in the 50 μg/ml bisphenol A-treated group compared with the control and the 0.5 μg/ml bisphenol A-treated group. The existence of ERα and β in the testis suggests that estrogens directly affect germ cells during testicular development and spermatogenesis, and differential modulation of ERα and β in the testis could be involved in the effects of bisphenol A.