- 作者：Hironori Koga ; M.D. ; Ph.D.&lowast ; ; Satoshi Hagiwara ; M.D. ; Ph.D.&lowast ; ; saku@oita-u.ac.jp ; Masafumi Inomata ; M.D. ; Ph.D.&dagger ; ; Jyunya Kusaka ; M.D.&lowast ; ; Nobuhiko Asai ; M.D.&lowast ; ; Masayoshi Oyama ; M.D.&lowast ; ; Kanako Kita ; M.D.&lowast ; ; Kenji Kashima ; M.D. ; Ph.D.&Dagger ; ; Isao Yokoi ; M.D. ; Ph.D.§ ; ; Takayuki Noguchi ; M.D. ; Ph.D.&lowast ;
- 关键词：vitamin E ; glutathione ; liver ; ischemic reperfusion ; cytokine
- 刊名：Journal of Surgical Research
- 出版年：2012
- 期刊代码：299_00224804
- 类别：med
- 出版时间：1 June, 2012
- 卷：175
- 期：1
- 页码：118-122
- 文件大小：723 K
Background
Ischemic liver injury is often the result of surgical procedures such as liver transplantation and hepatic resection. Liver damage occurs after reperfusion, leading to increased systemic inflammation. Recent studies have reported that vitamin E and glutathione can ameliorate ischemia-reperfusion (I/R) injury. In the present study, we evaluated the ability of a new vitamin E derivative, ETS-GS, to improve liver I/R injury.Materials and Methods
Male Wistar received a subcutaneous injection of ETS-GS (10 mg/kg) or saline before experimentally-induced liver I/R injury or sham treatment. The rats were sacrificed after the 60-min ischemia and 24-h reperfusion. Histology and serum levels of cytokines [tumor necrosis factor (TNF)-伪, interleukin (IL)-6, and high-mobility group box 1 (HMGB1) protein] and liver enzymes were determined to evaluate the protective effects of ETS-GS.
Results
We found that ETS-GS treatment attenuated I/R-induced histologic alterations, reduced levels of liver enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH). In addition, ETS-GS treatment decreased serum cytokine levels.
Conclusions
Taken together, our results demonstrate that ETS-GS attenuates I/R injury in a rat model and suggests that ETS-GS may exert anti-inflammatory effects. Accordingly, ETS-GS may have therapeutic potential to treat various clinical conditions involving I/R injury.