Immobilization of Aspergillus terreus on loofa sponge for soyasapogenol B production from soybean saponin

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• 作者：Hala Abdel Salam Amin ; ^{halaamin2007@yahoo.com} ; Sayeda Saleh Mohamed
• 关键词：Biotransformation ; Soybean saponin ; Soyasapogenol B ; A. terreus ; Immobilization
• 刊名：Journal of Molecular Catalysis B ; Enzymatic
• 出版年：2012
• 期刊代码：55_13811177
• 类别：ce
• 出版时间：June, 2012
• 卷：78
• 期：Complete
• 页码：85-90
• 文件大小：476 K

摘要

Soyasapogenol B is known to have hepatoprotective, antimutagenic, antivirus, and anti-inflammatory activities. Among five porous carriers tested for immobilization of Aspergillus terreus II for soyasapogenol B production from soybean saponin, loofa sponge was the best immobilization carrier. Best yield of 83.64%soyasapogenol B was reached with six loofa disks loaded with 10⁷ cells/mL spore concentration after 72 h in the first cycle. Effective soyasapogenol B yield was also observed using immobilized loofa disks in successive fermentations up to 8 batches and the highest yield (86.3%) was achieved on the third batch. Compared to free cell fermentation, soyasapogenol B productivity produced by immobilized loofa disks was 51.77%higher (64.2 mg/L h vs. 42.3 mg/L h), fermentation time was 33%lower (48 h vs. 72 h) and fermentation medium contents were reduced to half. Immobilization of A. terreus on loofa sponge, therefore, permits repeated reuse under the specified fermentation conditions for soyasapogenol B production.