Identification of hydroxy fatty acids by liquid chromatography–atmospheric pressure chemical ionization mass spectroscopy in Euglena gracilis
- 作者：Santiago-Vá ; zquez ; Lory Z. ; Mydlarz ; Laura D. ; Pavlovich ; James G. ; Jacobs ; Robert S.
- 关键词：AA ; arachidonic acid ; APCI ; atmospheric pressure chemical ionization ; HEDE ; hydroxyeicosadienoic acid ; HEPE ; hydroxyeicosapentaenoic acid ; HETE ; hydroxyeicosatetraenoic acid ; HODE ; hydroxyoctadecadienoic acid ; HOTrE ; hydroxyoctadecatrienoic acid ; HpEPE ; h
- 刊名：Journal of Chromatography B ; Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2004
- 期刊代码：124_15700232
- 类别：ch
- 出版时间：April 25, 2004
- 卷：803
- 期：2
- 页码：233-236
- 文件大小：74.4 K
摘要
Hydroxy fatty acids from Euglena gracilis were identified by reverse-phase high performance liquid chromatography coupled to a mass spectrometer run in atmospheric pressure chemical ionization positive ion mode. These metabolites were converted to methyl esters to improve stability and chromatographic properties. A detection limit of 20pg/μl per injection was determined for 5-HETE methyl ester based on the signal to noise ratio of the m/z 317 ion which corresponds to the loss of a hydroxyl group (M-17) and the major fragment in all HETE methyl esters studied. This is the first report for these metabolites in E. gracilis.