Single nucleotide mismatch analysis using oligonucleotide probes synthesized on bacterial magnetic particle

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• 作者：Ota ; Hiroyuki ; Arakaki ; Atsushi ; Tanaka ; Tsuyoshi ; Takeyama ; Haruko ; Matsunaga ; Tadashi
• 关键词：Bacterial magnetic particles ; Mismatch discrimination ; Oligonucleotide synthesis ; Hybridization
• 刊名：Biomolecular Engineering
• 出版年：2003
• 期刊代码：13_13890344
• 类别：imm
• 出版时间：July, 2003
• 卷：20
• 期：4-6
• 页码：305-309
• 文件大小：304 K

摘要

An approach to analyze mismatches using short and specific oligonucleotide probes directly synthesized on bacterial magnetic particles (BMPs) by phosphoramidite methods was exploited. Approximately 126 molecules of 4-mer oligonucleotides/particle were synthesized on BMPs with high reaction efficiencies. Hybridization between FITC-labeled oligonucleotides and chemically synthesized oligonucleotides on BMPs was performed. Perfect matched and mismatched hybridizations were successfully discriminated by using the oligonucleotide probes on BMPs.