High-cholesterol feeding aggravates cerebral infarction via decreasing the CB₁ receptor

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• 作者：Kazuhide Hayakawa ; Kenichi Mishima ; Masanori Nozako ; Mai Hazekawa ; Yo Aoyama ; Ayumi Ogata ; Kazuhiko Harada ; Masayuki Fujioka ; Kohji Abe ; Nobuaki Egashira ; et al.
• 关键词：Cerebral ischemia ; High-cholesterol ; Caloric restriction ; CB₁ receptor
• 刊名：Neuroscience Letters
• 出版年：2007
• 期刊代码：52_03043940
• 类别：neu
• 出版时间：6 March 2007
• 卷：414
• 期：2
• 页码：183-187
• 文件大小：363 K

摘要

We examined how feeding conditions affect the CB₁ receptor and cerebral infarction caused by cerebral ischemia. Mice were divided into the following three groups: normal diet (ND), caloric restriction (CR) and high-cholesterol-enriched diet (HCD), and were kept for 6 weeks. After 6 weeks, we measured both serum and brain cholesterol and the expression level of cannabinoid CB₁ receptor within the brain in intact mice. In addition, middle cerebral artery (MCA) was occluded for 2 h following reperfusion. Serum cholesterol significantly increased in the HCD group in comparison with both the ND and CR groups. However, brain cholesterol decreased in the HCD group. Then, the expression level of CB₁ receptor significantly decreased in the HCD group, while that of the CR group clearly increased in comparison with the ND group in intact mice. In MCA-occluded mice, The HCD group produced the most severe cerebral infarction, while cerebral infarction was significantly decreased in the CR group. These results suggest that CR prevents infarction by increasing CB₁ receptor expression, while high-cholesterol feeding aggravates cerebral infarction both by hypercholesterolemia in serum and by decreasing CB1 receptor expression modulated by hypocholesterolemia within the brain.