The antimicrobial peptide, epinecidin-1, mediates secretion of cytokines in the immune response to bacterial infection in mice
- 作者：Shang-Chun Lee ; Chieh-Yu Pan ; Jyh-Yih Chen ; zoocjy@gate.sinica.edu.tw
- 关键词：Epinecidin-1 ; Immune response ; Bacterial infection ; Cytokines
- 刊名：Peptides
- 出版年：2012
- 期刊代码：59_01969781
- 类别：neu
- 出版时间：July, 2012
- 卷：36
- 期：1
- 页码：100-108
- 文件大小：1335 K
摘要
Epinecidin-1, an antimicrobial peptide which encodes 21 amino acids, was isolated from a marine grouper (Epinephelus coioides). In this study, we investigated its immunomodulatory functions in mice co-injected with Pseudomonas aeruginosa. In vivo results showed that the synthetic epinecidin-1 peptide induced significant secretion of immunoglobulin G1 (IgG1) in mice co-injected with P. aeruginosa. Moreover, after injection of 40, 100, 200, or 500 渭g epinecidin-1/mouse, we detected IgM, IgG, IgG1, and IgG2a in mice treated for 1, 2, 3, 7, 14, 21, and 28 days. Results showed that there were no significant differences in IgM, IgG, or IgG2a between mice injected with epinecidin-1 alone. IgG1 increased to a peak at 24 h, 7 days, and 28 days after an epinecidin-1 (40 渭g/mouse) injection. Injection of 500 渭g epinecidin-1/mouse increased IgG1 to peaks at 2 and 3 days; injection of 100 渭g epinecidin-1/mouse increased IgG1 to a peak at 21 days. This supports epinecidin-1 being able to activate the Th2 cell response (enhance IgG1 production) against P. aeruginosa infection. Treatment with different concentrations of epinecidin-1 in mice elevated plasma interleukin (IL)-10 to initial peaks at 24 and 48 h, and it showed a second peak at 16 days. In RAW264.7 cells, treatment with epinecidin-1 alone did not produce significant changes in tumor necrosis factor (TNF)-伪 protein secretion at 1, 6, or 24 h after treatment with 3.75, 7.5, or 15 渭g/ml epinecidin-1 compared to the lipopolysaccharide group.