Structure-dependent efficacy of infectious bursal disease virus (IBDV) recombinant vaccines
- 作者：Martinez-Torrecuadrada ; Jorge L. ; Saubi ; Narcí ; s ; Pagè ; s-Manté ; Albert ; et. al.
- 关键词：Infectious bursal disease virus ; Baculovirus-expressed structures ; Avian vaccine ; IBDV
- 刊名：Vaccine
- 出版年：2003
- 期刊代码：89_0264410x
- 类别：imm
- 出版时间：July 4, 2003
- 卷：21
- 期：23
- 页码：3342-3350
- 文件大小：321 K
摘要
The immunogenicity and protective capability of several baculovirus-expressed infectious bursal disease virus (IBDV)-derived assemblies as VP2 capsids, VPX tubules and polyprotein (PP)-derived mixed structures, were tested. Four-week-old chickens were immunised subcutaneously with one dose of each particulate antigen. VP2 icosahedral capsids induced the highest neutralising response, followed by PP-derived structures and then VPX tubules. All vaccinated animals were protected when challenged with a very virulent IBDV (vvIBDV) isolate, however the degree of protection is directly correlated with the levels of neutralising antibodies. VP2 capsids elicited stronger protective immunity than tubular structures and 3μg of them were sufficient to confer a total protection comparable to that induced by an inactivated vaccine. Therefore, VP2 capsids represent a suitable candidate recombinant vaccine instead of virus-like particles (VLPs) for IBDV infections. Our results also provide clear evidence that the recombinant IBDV-derived antigens are structure-dependent in order to be efficient as vaccine components.