Use of intestinal Pseudomonas aeruginosa in fish to detect the environmental pollutant benzo[a]pyrene

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• 作者：Ali Karami^a ; ^{alikaramiv@gmail.com} ; Annie Christianus^a ; Zamri Ishak^b ; Zulkifli Hj. Shamsuddin^c ; Majid Masoumian^d ; Simon C. Courtenay^e
• 关键词：7 ; 8-D BaP ; 7 ; 8dihydrodiolbenzo[a]pyrene ; 1-OH BaP ; 1-hydroxybenzo[a]pyrene ; 3-OH BaP ; 3-hydroxybenzo[a]pyrene ; EROD ; ethoxyresorufin-O-deethylase ; GST ; glutathione-S-transferase ; FACs ; fluorescent aromatic compounds
• 刊名：Journal of Hazardous Materials
• 出版年：2012
• 期刊代码：50_03043894
• 类别：ce
• 出版时间：15 May, 2012
• 卷：215-216
• 期：Complete
• 页码：108-114
• 文件大小：369 K

摘要

This study examined the potential of Pseudomonas aeruginosa abundance in the intestines of fish as an indicator of exposure to benzo[a]pyrene (BaP). P. aeruginosa populations were enumerated in juvenile African catfish (Clarias gariepinus) injected intramuscularly three days previous with 0, 10, 30, 40, 50 or 70 mg/kg of BaP. Hepatic EROD and GST activities and biliary fluorescent aromatic compounds (FACs) 1-OH BaP, 3-OH BaP, 7,8-D BaP and BaP were quantified to investigate agreements between the new indicator and established fish biomarkers. The shape of bacterial population (logarithm of colony-forming unit) dose-response curve generally matched those of biliary FACs concentrations. Conversely, the EROD and GST dose-response curves were generally the mirror images of the bacterial population curve. Changes in intestinal P. aeruginosa population appear to be an indirect effect of BaP exposure because exposure to 0-100 渭g/ml BaP had no effect on P. aeruginosa populations grown on agar plates containing BaP. Using intestinal P. aeruginosa population of fish as a universal indicator of BaP pollution in aquatic environments is discussed.Conversely, the EROD and GST dose-response curves were generally the mirror images of the bacterial population curve.