Involvement of the Mycobacterium tuberculosis secreted antigen SA-5K in intracellular survival of recombinant Mycobacterium smegmatis
- 作者：Batoni ; Giovanna ; Bottai ; Daria ; Maisetta ; Giuseppantonio ; Pardini ; Manuela ; Boschi ; Antonella ; et. al.
- 关键词：Secreted protein ; Intracellular survival ; Mycobacterium tuberculosis ; Mycobacterium smegmatis
- 刊名：FEMS Microbiology Letters
- 出版年：2001
- 期刊代码：34_03781097
- 类别：imm
- 出版时间：November 27, 2001
- 卷：205
- 期：1
- 页码：125-129
- 文件大小：249 K
摘要
A new protein (SA-5K) secreted in culture filtrates by Mycobacterium bovis, Mycobacterium tuberculosis, and few other mycobacterial species was previously identified and purified in our laboratory. In order to evaluate the putative role of SA-5K during intracellular mycobacterial growth, in the present study the SA-5K gene was cloned and expressed in Mycobacterium smegmatis, a rapid growing non-pathogenic mycobacterium which does not contain the gene for the protein. SA-5K expression in the THP-1 human macrophage cell line infected with the recombinant strain (M. smegmatis-pROL5K) was demonstrated by RT-PCR on RNA extracted from bacterial cells following 24 and 48 h of infection. Intracellular SA5K expression was associated with a higher cfu increase of M. smegmatis-pROL5K in comparison to the negative control strain (M. smegmatis recombinant for the cloning vector) (P