Identification and molecular cloning of a novel secretion antigen from Mycobacterium tuberculosis and Mycobacterium bovis BCG
- 作者：Freer ; G. ; Florio ; W. ; Dalla Casa ; B. ; Bottai ; D. ; Batoni ; G. ; Maisetta ; G. ; Senesi ; S. ; Campa ; M.
- 关键词：Mycobacterium tuberculosis ; BCG ; Protein SA-5K ; Secretion antigens ; Molecular cloning
- 刊名：Research in Microbiology
- 出版年：1998
- 期刊代码：75_09232508
- 类别：imm
- 出版时间：April, 1998
- 卷：149
- 期：4
- 页码：265-275
- 文件大小：796 K
摘要
A novel protein called SA-5K was identified in Mycobacterium bovis BCG (BCG) short-term culture filtrates (CFs) by means of a recently described monoclonal antibody (mAb), L8D8. This protein had an apparent molecular mass (MM) of 5 kDa, as judged by Western blotting after sodium dodecyl sulphate-polyacrylamide gel electrophoresis in reducing conditions, and did not seem to contain any sugar or lipid substituents.