Application of a statistical design to the optimization of parameters and culture medium for α-amylase production by Aspergillus oryzae CBS 819.72 grown on gruel (wheat grinding by-product)

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• 作者：Radhouane Kammoun ; Belgacem Naili ; Samir Bejar
• 关键词：Aspergillus oryzae ; ttp ; //www.sciencedirect.com/scidirimg/entities/204e.gif" alt="greek small letter alpha" title="greek small letter alpha" border="0">-Amylase ; Box– ; Behnken design ; Plackett– ; Burman design ; Taguchi design
• 刊名：Bioresource Technology
• 出版年：2008
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：September 2008
• 卷：99
• 期：13
• 页码：5602-5609
• 文件大小：181 K

摘要

The production optimization of ttp://www.sciencedirect.com/scidirimg/entities/204e.gif" alt="greek small letter alpha" title="greek small letter alpha" border="0">-amylase (E.C.3.2.1.1) from Aspergillus oryzae CBS 819.72 fungus, using a by-product of wheat grinding (gruel) as sole carbon source, was performed with statistical methodology based on three experimental designs. The optimisation of temperature, agitation and inoculum size was attempted using a Box–Behnken design under the response surface methodology. The screening of nineteen nutrients for their influence on ttp://www.sciencedirect.com/scidirimg/entities/204e.gif" alt="greek small letter alpha" title="greek small letter alpha" border="0">-amylase production was achieved using a Plackett–Burman design. KH₂PO₄, urea, glycerol, (NH₄)₂SO₄, CoCl₂, casein hydrolysate, soybean meal hydrolysate, MgSO₄ were selected based on their positive influence on enzyme formation.