To determine the relationship between reduced susceptibility to biocides and the carriage of antiseptic resistance genes, cepA, qac螖E and qacE, as well as identifying the role of efflux pumps in conferring reduced susceptibility.
Susceptibility was assessed for five biocides: chlorhexidine, benzalkonium chloride, Trigene, MediHex-4, Mediscrub; and for 11 antibiotics against 64 isolates of Klebsiella pneumoniae. Susceptibility to all compounds was tested by the agar double dilution method (DDM) and the effect of efflux pumps on biocides determined by repeating the susceptibility studies in the presence of the efflux pump inhibitor carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The presence of the cepA, qac螖E and qacE genes was identified by polymerase chain reaction.
The bacteria were not widely antibiotic resistant though a few showed reduced susceptibility to cefoxitin, chloramphenicol and rifampicin and later-generation cephalosporins but not to carbapenems. Biocide susceptibility, tested by DDM, showed that 50, 49 and 53 strains had reduced susceptibility to chlorhexidine, Trigene and benzalkonium chloride, respectively. The antiseptic resistance genes cepA, qac螖E and qacE were found in 56, 34 and one isolates respectively and their effects as efflux pumps were determined by CCCP (10聽mg/L), which decreased the minimum inhibitory concentrations (MICs) of chlorhexidine and Medihex-4 by 2-128-fold but had no impact on the MICs of benzalkonium chloride, Trigene and Mediscrub.
There was a close link between carriage of efflux pump genes, cepA, qac螖E and qacE genes and reduced biocide susceptibility, but not antibiotic resistance, in K.聽pneumoniae clinical isolates.