Identification of a permease gene involved in lactose utilisation in Aspergillus nidulans

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• 作者：Erzsé ; bet Fekete^a ; Levente Karaffa^a ; Bernhard Seiboth^b ; É ; va Fekete^a ; Christian P. Kubicek^b ; Michel Flipphi^a ; ^c ; ^{flipphi@iata.csic.es}
• 关键词：Lactose permease ; Intracellular 尾-galactosidase ; Gene cluster ; Regulation of transcription ; Lactose catabolism ; Multi-component lactose uptake
• 刊名：Fungal Genetics and Biology
• 出版年：2012
• 期刊代码：116_10871845
• 类别：abs
• 出版时间：June, 2012
• 卷：49
• 期：6
• 页码：415-425
• 文件大小：685 K

摘要

Lactose is intracellularly hydrolysed by Aspergillus nidulans. Classical mutation mapping data and the physical characteristics of the previously purified glycosyl hydrolase facilitated identification of the clustered, divergently transcribed intracellular 尾-galactosidase (bgaD) and lactose permease (lacpA) genes. At the transcript level, bgaD and lacpA were coordinately expressed in response to d-galactose, lactose or l-arabinose, while no transcription was detectable in the additional presence of glucose. In contrast, creA loss-of-function mutants derepressed for both genes to a considerable extent (even) under non-inducing or repressing growth conditions. Lactose- and d-galactose induction nevertheless occurred only in the absence of glucose, indicating a regulatory role for CreA-independent repression. Remarkably, bgaD deletion mutants grew normal on lactose. In contrast, lacpA deletants grew at a much slower rate in lactose liquid medium than wild-type while strains that carried more than one copy of lacpA grew faster, showing that transport is the limiting step in lactose catabolism. The effect of lacpA gene deletion on lactose uptake was exacerbated at lower substrate concentrations, evidence for the existence of a second transport system with a lower affinity for this disaccharide in A. nidulans.