Improvement of hydrogen production by transposon-mutagenized strain of Pantoea agglomerans BH18

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• 作者：Hongyan Liu^a ; Guangce Wang^a ; ^b ; ^{gcwang@qdio.ac.cn} ; Daling Zhu^a ; Guanghua Pan^a
• 关键词：Transposon ; Tn7 ; Hydrogen production ; Pantoea agglomerans BH18
• 刊名：International Journal of Hydrogen Energy
• 出版年：2012
• 期刊代码：42_03603199
• 类别：ch
• 出版时间：May, 2012
• 卷：37
• 期：10
• 页码：8282-8287
• 文件大小：413 K

摘要

Pantoea agglomerans BH18, isolated from mangrove sludge, could produce hydrogen under marine culture condition. To improve the hydrogen-producing capacity of this strain, we constructed a stable transposon-mutagenized library of P. agglomerans BH18. A Tn7-based transposon was randomly inserted into genomic DNA of P. agglomerans BH18. Mutants were identified by kanamycin resistance and amplification of the inserted transposon sequences. A transposon mutant, named as strain TB212, was screened for the highest hydrogen production ability. The total volume of hydrogen gas evolved by this mutant strain TB212 was 60%higher than that of the wild type. The mutant strain TB212 was able to produce hydrogen over a wide range of initial pH from 5.0 to 10.0, with an optimum initial pH of 7.0, and hydrogen production was 2.52聽卤聽0.02聽mol H₂/mol glucose (mean聽卤聽S.E.) under marine culture condition. The mutant strain TB212 could produce hydrogen at the salt concentration from 3 to 6%. It was concluded that the transposon-mutagenized library may be a useful tool for investigation of high efficiency hydrogen-producing bacteria.