Simultaneous determination of citrus limonoid aglycones and glucosides by high performance liquid chromatography
- 作者：Amit Vikram ; G.K. Jayaprakasha ; Bhimanagouda S. Patil
- 关键词：Liquid chromatography ; Method development ; Limonoid aglycones ; Limonoid glucosides
- 刊名：Analytica Chimica Acta
- 出版年：2007
- 期刊代码：2_00032670
- 类别：ch
- 出版时间：8 May 2007
- 卷：590
- 期：2
- 页码：180-186
- 文件大小：624 K
摘要
High performance liquid chromatography (HPLC) method has been developed for simultaneous quantification of limonoid aglycones and glucosides on a reversed phase C<sub>18sub> column using a binary solvent system, coupled with diode array detector. Seven limonoids such as limonin, nomilin, isolimonic acid, ichangin, isoobacunoic acid, limonin 17-β-d glucopyranoside and deacetyl nomilinic acid 17-β-d glucopyranoside were separated and detected at 210 nm. Furthermore, limonoids were separated, identified and quantified in four varieties of citrus fruits and seeds using developed method. Limonin and limonin glucoside were found to be the predominant limonoid aglycone and glucoside, respectively, in all tested samples. The sensitivity of the method was found to be 0.25–0.50 μg for tested limonoids.