Limonin 7-methoxime interferes with Escherichia coli biofilm formation and attachment in type 1 pili and antigen聽43 dependent manner
- 作者：Amit Vikram<sup>asup><sup> ; sup><sup>1sup> ; Guddadarangavvahally K. Jayaprakasha<sup>asup> ; Palmy R. Jesudhasan<sup>bsup> ; Suresh D. Pillai<sup>asup><sup> ; sup><sup>bsup> ; Bhimanagouda S. Patil<sup>asup><sup> ; sup><sup>b-patil@tamu.edusup>
- 关键词：Limonin ; Limonin 7-methoxime ; Limonin 7-oxime ; De-furan limonin ; Citrus ; Limonoids ; Cell&ndash ; cell signaling ; Quorum sensing ; Adhesins
- 刊名：Food Control
- 出版年：2012
- 期刊代码：49_09567135
- 类别：abs
- 出版时间：August, 2012
- 卷：26
- 期：2
- 页码：427-438
- 文件大小：949 K
摘要
Citrus limonoids were reported to inhibit autoinducer-mediated bacterial cell-cell signaling, Escherichia coli O157:H7 biofilms and the Type III secretion system. The goal of the present study was to understand the effect of specific modifications in limonin molecule on cell-cell signaling inhibitory activity. The limonin was modified into de-furan limonin, limonin 7-oxime, limonin 7-methoxime. The modified limonoids were examined for their cell-cell signaling and biofilm inhibitory properties. The results of the study suggest that the furan ring and C-7 substitutions were important structural features in the limonin molecule to inhibit cell-cell signaling and biofilm formation in Vibrio harveyi. The C-7 modifications of limonin showed increased potency to inhibit the E.聽coli biofilm. Furthermore, the data indicates that limonin聽7-methoxime reduced the attachment of E.聽coli to plastic surface by interacting with type 1 pili and Ag43. In addition, limonin 7-methoxime seems to reduce the adhesion of E.聽coli to spinach leaves and colon epithelial cells.