Enterotoxins and emetic toxins production by Bacillus cereus and other species of Bacillus isolated from Soumbala and Bikalga, African alkaline fermented food condiments
- 作者：Labia Irene I. Ouoba ; Line Thorsen ; Alan H. Varnam
- 关键词：Soumbala ; Bikalga ; Bacillus ; Enterotoxins ; Cereulide ; Starter cultures ; Probiotic bacteria
- 刊名：International Journal of Food Microbiology
- 出版年：2008
- 期刊代码：63_01681605
- 类别：abs
- 出版时间：10 June 2008
- 卷：124
- 期：3
- 页码：224-230
- 文件大小：199 K
摘要
The ability of various species of Bacillus from fermented seeds of Parkia biglobosa known as African locust bean (Soumbala) and fermented seeds of Hibiscus sabdariffa (Bikalga) was investigated. The study included screening of the isolates by haemolysis on blood agar, detection of toxins in broth and during the fermentation of African locust bean using the Bacillus cereus Enterotoxin Reverse Passive Latex Agglutination test kit (BCET-RPLA) and the Bacillus Diarrhoeal Enterotoxin Visual Immunoassay (BDEVIA). Detection of genes encoding cytotoxin K (CytK), haemolysin BL (Hbl A, Hbl C, Hbl D), non-hemolytic enterotoxin (NheA, NheB, NheC) and EM1 specific of emetic toxin producers was also investigated using PCR with single pair and multiplex primers.