Formation of chitin-based nanomaterials using a chitin-binding peptide selected by phage-display

详细信息	查看全文 | 推荐本文 |

• 作者：Feisal Khoushab^a ; ¹ ; Nanthnit Jaruseranee^a ; ¹ ; Waraporn Tanthanuch^b ; Montarop Yamabhai^a ; ^{montarop@sut.ac.th} ; ^{montarop@gmail.com}
• 关键词：Phage display ; Peptide ; Chitin ; Nanomaterial ; Nanobiotechnology
• 刊名：International Journal of Biological Macromolecules
• 出版年：2012
• 期刊代码：62_01418130
• 类别：abs
• 出版时间：1 June, 2012
• 卷：50
• 期：5
• 页码：1267-1274
• 文件大小：1096 K

摘要

Targeting polymers with peptides is an efficient strategy to functionalize biomaterials. Phage display technology is one of the most powerful techniques for selecting specific peptides for a wide variety of targets. A method to select a chitin-binding peptide from a 12-mer random peptide library was successfully performed against chitin immobilized in wells of microtiter plates. The synthetic chitin binding peptide (ChiBP) could bind to chitin beads and disrupt their structure. This selected peptide was successfully used to immobilize alkaline phosphatase on chitin. In addition, the peptide could induce colloidal chitin in water to form a chitin coat on the surface of plastic tubes. Scanning electron microscopy (SEM) revealed that the peptide could induce colloidal chitin and chitohexaose to form networks when the temperature was raised to 42 掳C.