The modulation of hepatic adenosine triphosphate and inflammation by eicosapentaenoic acid during severe fibrotic progression in the SHRSP5/Dmcr rat model

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• 作者：Xiaofang Jia^a ; Hisao Naito^a ; Husna Yetti^a ; Hazuki Tamada^a ; ^b ; Kazuya Kitamori^a ; ^b ; Yumi Hayashi^a ; Nozomi Yamagishi^a ; Dong Wang^a ; Yukie Yanagiba^a ; Yuki Ito^c ; Juncai Wang^a ; Naoki Tanaka^d ; Katsumi Ikeda^e ; Yukio Yamori^f ; Tamie Nakajima^a ; ^{tnasu23@med.nagoya-u.ac.jp}
• 关键词：Eicosapentaenoic acid ; High fat-cholesterol diet ; Fibrotic steatohepatitis ; Nuclear factor-魏B ; Fatty acid 尾-oxidation ; Adenosine triphosphate
• 刊名：Life Sciences
• 出版年：2012
• 期刊代码：62_00243205
• 类别：imm
• 出版时间：14 June, 2012
• 卷：90
• 期：23-24
• 页码：934-943
• 文件大小：1447 K

摘要

Aims

Eicosapentaenoic acid (EPA) can ameliorate certain liver lesions involved in non-alcoholic steatohepatitis (NASH). A previous study has found that stroke-prone spontaneously hypertensive 5/Dmcr (SHRSP5/Dmcr) rats fed a high fat-cholesterol (HFC) diet developed fibrotic steatohepatitis with histological similarities to NASH. This study evaluated the potential effects and mechanisms of action of EPA supplementation using this rodent model.

Main methods

Male rats were randomly assigned to groups that were fed with either the stroke-prone (SP) diet or HFC diet with or without EPA for 2, 8 and 14 weeks, respectively. The liver histopathology, biochemical features, mRNA and protein levels, and nuclear factor-魏B (NF-魏B) DNA binding activity were determined.

Key findings

The SP diet-fed rats presented normal livers. Conversely, the HFC diet-fed rats developed microvesicular/macrovesicular steatosis, inflammation, ballooning degeneration and severe fibrosis. At 2 weeks, the administration of EPA inhibited hepatic inflammatory recruitment by blocking the phosphorylation of inhibitor of 魏B-伪 (I魏B伪), which antagonizes the NF-魏B activation pathway. The dietary supplementation of EPA for 8 weeks ameliorated hepatic triglyceride accumulation and macrovesicular steatosis by inhibiting the HFC diet-induced decrease in the protein levels of enzymes involved in fatty acid 尾-oxidation including carnitine palmitoyltransferase 1, very long chain acyl-CoA dehydrogenase and peroxisomal bifunctional protein. Although the administration of EPA elicited no histologically detectable effects on severe fibrosis at 14 weeks, it restored an HFC diet-induced decline in hepatic adenosine triphosphate (ATP) levels and suppressed ballooning degeneration, suggesting that EPA may inhibit HFC diet-induced ATP loss and cell death.

Significance

Initial amelioration of the inflammation and steatosis in the rats after EPA supplementation indicates a possibility to treat steatohepatitis. Additionally, this study provides new insights into the roles of EPA in hepatic ATP depletion and subsequent hepatocellular injury during severe fibrosis.