Development of an immunochromatographic strip test for the rapid simultaneous detection of deoxynivalenol and zearalenone in wheat and maize

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• 作者：Zhi-Bing Huang^a ; Yang Xu^a ; ^{xuyang1951@163.com} ; ^{xuyang1951@yahoo.com.cn} ; Lai-Sheng Li^b ; Yan-Ping Li^a ; Hong Zhang^a ; Qing-Hua He^a
• 关键词：Colloidal gold ; Immunochromatographic strip ; Simultaneous detection ; Deoxynivalenol ; Zearalenone ; Wheat ; Maize
• 刊名：Food Control
• 出版年：2012
• 期刊代码：49_09567135
• 类别：abs
• 出版时间：November, 2012
• 卷：28
• 期：1
• 页码：7-12
• 文件大小：769 K

摘要

A colloidal gold immunochromatographic strip (ICS) test based on competitive format was developed for the rapid simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEN) in wheat and maize samples. For gold-based ICS test, antigen (DON-CBSA and ZEN-BSA) and goat anti-mouse IgG were respectively drawn on NC membrane as two test line (T₁ and T₂ line) and control line (C line), respectively. Monoclonal antibody (MAb)-gold conjugates (anti-DON MAb-gold and anti-ZEN MAb-gold) were sprayed onto the conjugate pad. To perform the test, 5聽g of sample was extracted in a ratio of 1:5 with 50%methanol-water (v/v) by shaking for 3聽min and the extract directly used without further cleanup steps. Matrix interference was eliminated by 2.5-fold dilutions of water extracts with buffer. The ICS test, which has cut-off levels of 100聽ng/mL (1000聽渭g/kg) and 6聽ng/mL (60聽渭g/kg) for DON and ZEN, respectively, and can be completed in 5聽min. Analysis of DON and ZEN in wheat and maize samples revealed that data obtained from ICS test were in a good agreement with those obtained from ELISA and instrumental analysis (GC/MS for DON determination and HPLC for ZEN determination). The results demonstrate that the ICS test can be used as a reliable, rapid, cost-effective and convenient qualitative tool for on-site screening technique for the simultaneous determination of DON and ZEN in wheat and maize samples without special instrumentation.