A standardization of Amphibalanus (Balanus) amphitrite (Crustacea, Cirripedia) larval bioassay for ecotoxicological studies

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• 作者：Veronica Piazza^a ; ^{veronica.piazza@ge.ismar.cnr.it} ; Annalisa Ferioli^b ; Elisabetta Giacco^c ; Nunzia Melchiorre^d ; Alessandra Valenti^e ; Francesco Del Prete^f ; Francesca Biandolino^g ; Letizia Dentone^h ; Paola Frisendaⁱ ; Marco Faimali^a
• 关键词：Bioassay ; Standardization ; Amphibalanus amphitrite ; Interlaboratory comparison
• 刊名：Ecotoxicology and Environmental Safety
• 出版年：2012
• 期刊代码：77_01476513
• 类别：env
• 出版时间：1 May, 2012
• 卷：79
• 期：Complete
• 页码：134-138
• 文件大小：240 K

摘要

A series of 10 intra-laboratory bioassays and an inter-laboratory assay involving nine laboratories, were performed to validate the use of Amphibalanus amphitrite larvae as test organisms for ecotoxicological studies. The standardization protocol utilized Cadmium Nitrate as a reference toxicant and larval immobilization (after 24 and 48 h) as the end-point. The statistical data analysis showed high homogeneity in intra-laboratory EC₅₀ values among bioassay repetitions (coefficient of variation (CV)=15.8%after 24 h and 16.9%after 48 h); also CV values obtained in inter laboratory comparison exercise were not higher than the 50%, variation coefficient mentioned in the ISO standards for the precision of inter-laboratory assays. The results demonstrated that the protocol implemented for the balanus larval bioassay comply with the international standards both for intra- and inter-laboratory precision.