Enzyme-nanoparticle conjugates at oil-water interface for amplification of electrochemical immunosensing

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• 作者：Huilan Su ; Ruo Yuan ; ^{yuanruo@swu.edu.cn} ; Yaqin Chai ; Ying Zhuo
• 关键词：Biosensor ; Electrochemistry ; Catalytic capsule ; AFP
• 刊名：Biosensors and Bioelectronics
• 出版年：2012
• 期刊代码：12_09565663
• 类别：ch
• 出版时间：15 March, 2012
• 卷：33
• 期：1
• 页码：288-292
• 文件大小：681 K

摘要

In the current research, the preparation of biocatalytic capsule (Ab₂-catalytic capsule) was based on utilizing 鈥渙il鈥?(organic compound) to entrap the nano-Au/HRP/HAFP bioconjugates, which were produced by horseradish peroxidase (HRP) and HRP labeled anti-AFP (HAFP) co-conjugated on gold nanoparticles (nano-Au). For the first time, a signal amplification strategy was introduced by the prepared biocatalytic capsule as labels for electrochemical immunodetection of 伪-1-fetoprotein (AFP). The transmission electron microscopy (TEM) images indicated that the biocatalytic capsules were successfully synthesized. The cyclic voltammetry (CV) experiments provided the evidence that the prepared capsule exhibited high catalytic activity for the reduction of H₂O₂. Besides, a biocompatible monolayer (collagen-TiO₂) was introduced to immobilize a nano-Au layer to fabricate the primary antibody on the electrode surface. After the prepared biocatalytic capsule was captured to the electrode surface to form the sandwich type immunoassay, the enhanced sensitivity was obtained with a detection limit of 29 pg/mL. The proposed immunoassay also presented good stability, selectivity and storage stability.