Biochemical properties and thermal behavior of pectate lyase produced by Pectobacterium carotovorum subsp. carotovorum BR1 with industrial potentials

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• 作者：V.B. Maisuria ; A.S. Nerurkar ; ^{anuner26@yahoo.com}
• 关键词：PL ; pectate lyase ; PG ; polygalacturonase ; PNL ; pectin lyase ; Pcc ; Pectobacterium carotovorum subsp. carotovorum ; Ecc ; Erwinia carotovora subsp. carotovora ; Eca ; E. carotovora subsp. atroseptica ; Ech ; E. chrysanthemi ; w/v ; weight per volume ; 螖H# ; enthalpy
• 刊名：Biochemical Engineering Journal
• 出版年：2012
• 期刊代码：9_1369703x
• 类别：ce
• 出版时间：15 April, 2012
• 卷：63
• 期：Complete
• 页码：22-30
• 文件大小：563 K

摘要

An extracellular pectate lyase (PL, EC 4.2.2.2) produced by Pectobacterium carotovorum subsp. carotovorum BR1 was purified and characterized with respect to its biochemical properties including the thermodynamic parameters of substrates hydrolysis and thermal behavior. The PL showed higher affinity for polygalacturonic acid (K_m, 0.4 g l^鈭?) as compared to 70%methylated pectin (K_m, 0.77 g l^鈭?). Nonlinear thermal inactivation curves of PL at 50 and 60 掳C, fitted to a three-fraction first-order kinetic model. Furthermore, the thermodynamic parameters of thermal deactivation of purified PL viz. 螖H*, 螖S*, E_d and 螖G* were determined over pH range 6-10 and in presence of different cations. Alkaline pH and Ca²⁺ ions individually influenced the thermostability of PL at elevated temperatures 60 and 70 掳C, based on the thermodynamic parameters. The studies suggested that this alkaline PL can be considered as potential candidate for various applications like pectic waste management, degumming of fibers, food, paper and textile industries.