Proteomics and transcriptome analysis coupled with pharmacological test reveals the diversity of anti-thrombosis proteins from the medicinal insect, Eupolyphaga sinensis

详细信息	查看全文 | 推荐本文 |

• 作者：Ying Wang^a ; ^d ; ¹ ; Hongli Yan^b ; ¹ ; Yipeng Wang^a ; Hailong Yang^a ; Lin Wei^b ; Yao Xiao^a ; ^d ; Huahu Ye^c ; Ren Lai^a ; ^b ; ^{rlai@mail.kiz.ac.cn} ; Rui Liu^b ; ^{liuruisl@163.com}
• 关键词：Insect ; Thrombosis ; Plasminogen-activator ; Fibrin(ogen)olytic
• 刊名：Insect Biochemistry and Molecular Biology
• 出版年：2012
• 期刊代码：80_09651748
• 类别：bio
• 出版时间：August, 2012
• 卷：42
• 期：8
• 页码：537-544
• 文件大小：1846 K

摘要

The insect of Eupolyphaga sinensis Walker has been used as traditional anti-thrombosis medicine without bleeding risk for several hundreds years in eastern countries. Our previous work has identified a bi-functional anti-thrombosis protein containing both direct-acting fibrin(ogen)olytic and plasminogen-activating activities from the insect. By proteomics and transcriptome analysis, 105 serine proteases belonging to four families were identified from the ground beetle, E.聽sinensis and the classification is for serine proteases of this organism. Pharmacological test indicated that 5 (eupolytin 1-5) of them have the abilities to hydrolyze fibrin(ogen) and/or activate plasminogen. The current work revealed the extreme diversity of anti-thrombosis components in E.聽sinensis and anti-thrombosis molecular mechanisms of the traditional medicinal insect, and provided many templates for the development of new thrombolytic agents. Especially, these proteins, which contain both plasmin- and PA (plasminogen-activating)-like activities, are excellent candidates for anti-thrombosis medicines.