Inhibition of corneal inflammation following keratoplasty by birch leaf extract

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• 作者：Katrin Wacker^a ; ¹ ; ^{Katrin.Wacker@uniklinik-freiburg.de} ; Carsten Grü ; ndemann^b ; ¹ ; ^{Carsten.Gruendemann@uniklinik-freiburg.de} ; Yvonne Kern^a ; ^{Yvonne.Kern@uniklinik-freiburg.de} ; Laura Bredow^a ; ^{Laura.Bredow@uniklinik-freiburg.de} ; Roman Huber^b ; ^{Roman.Huber@uniklinik-freiburg.de} ; Thomas Reinhard^a ; ^{Thomas.Reinhard@uniklinik-freiburg.de} ; Johannes Schwartzkopff^a ; ^{Johannes.Schwartzkopff@uniklinik-freiburg.de}
• 关键词：keratoplasty ; corneal inflammation ; immunosuppression ; birch leaf
• 刊名：Experimental Eye Research
• 出版年：2012
• 期刊代码：293_00144835
• 类别：med
• 出版时间：April, 2012
• 卷：97
• 期：1
• 页码：24-30
• 文件大小：872 K

摘要

The objective of this study was to determine the effect of birch leaf (Betula pendula) extract (BPE) on corneal inflammation following keratoplasty in the rat model. T cells were stimulated in聽vitro in the presence of BPE. Proliferation, activation phenotype and the number of apoptotic/necrotic cells in cell culture were analyzed by flow cytometry. Corneal transplantation was performed between Fisher and Lewis rats. Recipient rats were either treated with cyclosporine A at a low dosage (Low-dose CsA = LDCsA) or received LDCsA in combination with BPE (2 脳 1 ml/day). Clinical signs for corneal inflammation and rejection time points were determined. Infiltrating leukocytes were analyzed histologically. BPE specifically inhibited T cell proliferation in聽vitro by inducing apoptosis. The phenotype was not affected. In聽vivo, BPE significantly delayed the onset of corneal opacification (p < 0.05). The amount of infiltrating CD45⁺ leukocytes and CD4⁺ T cells (p < 0.001) was significantly reduced by BPE, whereas infiltration of CD163⁺ macrophages was not significantly different between the two groups. BPE selectively induces apoptosis of activated T cells. Accordingly, BPE treatment significantly reduces infiltrating T cells and subsequent corneal opacification following keratoplasty. Our findings suggest BPE as a promising anti-inflammatory drug to treat corneal inflammation.