Different fungal manganese-oxidizing peroxidases: a comparison between Bjerkandera sp. and Phanerochaete chrysosporium

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• 作者：Palma ; C. ; Martí ; nez ; A.T. ; Lema ; J.M. ; Martí ; nez ; M.J.
• 关键词：Mn-oxidizing peroxidases ; Catalytic properties ; Bjerkandera sp. ; Phanerochaete chrysosporium
• 刊名：Journal of Biotechnology
• 出版年：2000
• 期刊代码：53_01681656
• 类别：imm
• 出版时间：February 17, 2000
• 卷：77
• 期：2-3
• 页码：235-245
• 文件大小：228 K

摘要

Two manganese-oxidizing peroxidases differing in glycosylation degree were purified from fermenter cultures of Bjerkandera sp. They were characterized and compared with the three manganese-oxidizing peroxidase isoenzymes obtained from the well-known ligninolytic fungus Phanerochaete chrysosporium. All the enzymes showed similar molecular masses but those from P. chrysosporium had less acidic isoelectric point. Moreover, the latter strictly required Mn²⁺ to oxidize phenolic substrates whereas the Bjerkandera peroxidases had both Mn-mediated and Mn-independent activity on phenolic and non-phenolic aromatic substrates. Taking into account these results, and those reported for Bjerkandera adusta and different Pleurotus species, we concluded that two different types of Mn²⁺-oxidizing peroxidases are secreted by ligninolytic fungi.