Evaluation of PAH bioaccumulation and DNA damage in mussels (Mytilus galloprovincialis) exposed to spilled Prestige crude oil
- 作者：P脙脙rez-Cadah脙脙a ; Beatriz ; Laffon ; Blanca ; P脙脙saro ; Eduardo ; M脙脙ndez ; Josefina
- 关键词：Mytilus galloprovincialis ; Prestige spill ; Comet assay ; Genotoxicity ; DNA damage ; Polycyclic aromatic hydrocarbons ; Benzo[a]pyrene ; Biodisponibility
- 刊名：Comparative Biochemistry and Physiology ; Part C
- 出版年：2004
- 期刊代码：8_15320456
- 类别：pha
- 出版时间：August, 2004
- 卷：138
- 期：4
- 页码：453-460
- 文件大小：257 K
摘要
We analyzed the hydrocarbon composition of the Prestige oil as it reached the shores, its solubility in sea water, its bioaccumulation, and the genotoxic damage associated to oil exposure, using Mytilus galloprovincialis as sentinel organism. Mussels were exposed to two oil volumetric ratios (1:500 and 2:500) for 12 days. Great concentrations of total polycyclic aromatic hydrocarbons (TPAH) have been obtained, being in general higher in the samples from the dose of 1:500, both in sea water (55.14 vs. 41.96 脦脦g/l) and mussel tissue (16,993.80 vs. 17,033.00 脦脦g/kg), probably due to the great tendency of these compounds to link to particles in water. Comet assay results reflected an increase in the DNA damage associated to oil exposure, higher in the mussels exposed to the higher aqueous TPAH content. In the view of our results, the importance of the evaluation of biodisponibility, bioaccumulation and DNA damage in the assessment of the effects of xenobiotic pollutants to marine environments could be highlighted.