⁶⁸Ga-Autoclabeling of DOTA-TATE and DOTA-NOC

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• 作者：Elisabeth Blom ; Jacek Koziorowski ; ^{jacek@heh.regionh.dk}
• 关键词：68Ga ; DOTA-TATE ; DOTA-NOC
• 刊名：Applied Radiation and Isotopes
• 出版年：2012
• 期刊代码：6_09698043
• 类别：ce
• 出版时间：June, 2012
• 卷：70
• 期：6
• 页码：980-983
• 文件大小：244 K

摘要

Introduction

A new method combining ⁶⁸Ga-labeling and steam sterilization, here called autoclabeling, has been evaluated for two somatostatin receptor binding tracers used for positron emission tomography (PET) imaging of neuroendocrine tumors; DOTA-TATE and -NOC.

Methods

The two peptides DOTA-TATE and -NOC were labeled with ⁶⁸Ga by heating for 15 min at 121 掳C in the presence of acetate buffer at pH 4.3. The product solutions were tested for sterility, presence of endotoxins, degradation of peptide and osmolality.

Results

Complete incorporation of ⁶⁸Ga was obtained after the autoclabeling reaction and no degradation of the peptides was observed. Sterility was verified and the presence of endotoxins was well within Ph. Eur limits (175IU/maximum injected volume).

Conclusions

The autoclabeling method provides a convenient procedure for ⁶⁸Ga-labeling by combining the labeling reaction and steam sterilization into one single step.