BMP4 localization and PCNA expression during distraction osteogenesis of the porcine mandible
- 作者：G.M. Hansen ; M.E. Lawler ; W.B. Williams ; M.J. Troulis ; L.B. Kaban ; lkaban@partners.org
- 关键词：mandibular distraction osteogenesis ; immunohistochemistry ; PCNA ; BMP4
- 刊名：International Journal of Oral and Maxillofacial Surgery
- 出版年：2012
- 期刊代码：149_09015027
- 类别：med
- 出版时间：July, 2012
- 卷：41
- 期：7
- 页码：867-873
- 文件大小：941 K
摘要
This study characterized sequential molecular and cellular events in the porcine mandibular distraction osteogenesis (DO) wound. Nineteen Yucatan minipigs were divided into three treatment groups: Group A, unilateral mandibular distraction with 0 day latency, 1 mm/day rate for 12 days, 24 days fixation (n = 16); Group B, acute lengthening 12 mm (n = 2); Group C, sham control (n = 1). Group A was further divided by death date: mid-DO (n = 5), end-DO (n = 4), mid-fixation (n = 5) and end-fixation (n = 2). Groups B and C were killed on postoperative day 36, corresponding to end-fixation. Specimens were stained for proliferating cell nuclear antigen (PCNA) and bone morphogenetic protein-4 (BMP4). Cellular proliferation (PCNA) was assessed quantitatively and BMP4 staining was assessed on a semi-quantitative scale. Progenitor cell proliferation was greatest during mid-DO and decreased from end-DO through end-fixation. Proliferation in the acute lengthening group was elevated relative to sham control and comparable to end-DO. BMP4 staining intensity (localized to the periosteal cambium layer) was greatest during mid- and end-DO, decreased at mid-fixation and was undetectable at end-fixation. Progenitor cell proliferation and BMP4 expression are greatest during mid-DO and decrease progressively thereafter. At the time of death of the acute lengthening group, only increased cell proliferation was demonstrated.